HSS 1100 Lecture Notes - Lecture 3: Immunofluorescence, Intracellular Ph, Osmotic Pressure

• School: University of Ottawa
• Department: Health Sciences
• Course: HSS 1100
• Professor: William Yan

Document Summary

Frozen at -80 c: preservation of pure cultures, wh(cid:455) (cid:449)ould (cid:449)e (cid:449)a(cid:374)t to keep a (cid:862)(cid:272)op(cid:455)(cid:863) of a (cid:271)a(cid:272)teria (cid:449)e isolated fro(cid:373) a patient, short term versus long-term. Identification: no(cid:449) that (cid:455)ou ha(cid:448)e a pure (cid:272)ulture . Cellular morphology: the microscope is your friend. Resolving power (resolution) = ability to distinguish two closely located objects as separate, distinct entities. Identification - staining techniques : generally, three steps, make a smear, fix dried smear by heat, stain with desired dye, simple vs. Size, shape, number, arrangement, etc: differential stain. Differences between microorganisms or parts of cells. Acid fast, gram: the gram stain (hans christian gram, flood slide with crystal (or gentian) violet. (wash with running tap water, flood (cid:449)ith gra(cid:373)"s iodine. (wash with water, carefully decolorize with 95% ethanol. (wash with water) This third step is the most critical and also the one most affected by technical variations in timing and reagents: flood with safranin (pink color). (wash with water).