BIO304H5 Lecture Notes - Lecture 16: Voltage Clamp, Reticular Theory, Pipette
Document Summary
Lecture 16 exam review: relied on squid synapse. Just like it has a giant axon, has a giant synapse. Formed by pre-synaptic neuron which comes from cns and activates a whole series of axons including in blue, the giant axon that goes on to innervate the mantle. Neurons are so big, can easily pole with electrodes and look at pre-and post-synaptic processes. Used to see how synapse works: applied ttx effective sodium channel blocker. Aps in pre-synaptic axons stimulate it and record ap in electrode. Did(cid:374)"t (cid:449)a(cid:374)t to thi(cid:374)k a(cid:271)out (cid:1005)st 2 where there was an. Ap in post-synaptic cell bc just cared about epsp. Epsps decline as a function of pre-synaptic ap amplitude: can see: as you increase amplitude, get an increase in post-synaptic response, a very strong depolarization (70mv) produces a strong epsp in the post-synaptic cell. (cid:449)hereas (cid:449)ith the sti(cid:373)ulati(cid:374)g ele(cid:272)trode, there"s (cid:374)o sodiu(cid:373) i(cid:374)(cid:448)ol(cid:448)ed.
