BIO370Y5 Lecture Notes - Lecture 7: Chromosome
Document Summary
Genetic recombination and complementation: good tools for mutant analysis. Adding two loci (on different or other pieces of dna) onto the same chromosome. Two homologous regions are cut, exchanged and than tapped (rejoined) Isolated mutants can provide info on how certain proteins act. Can help with finding the number of genes responsible for certain phenotype. Can help with distinguishing regulatory genes and regulatory dna sites. Need a partial diploid for all analysis methods. Eg: genetic recombination occurs when a two phages attack bacteria. Two chromosomes align and a cut is made. Only a few recombinant phages appear when bacteria is lysed. Exogenote fates: stabilization (what"s this?, degradation, recombination. In actual experiment, the donor does not survive. The piece of dna that is non-viable got its definition due to it"s linear nature. Linkage: measure the likelihood that two genes co-transfer together (closer = increased likelihood)
