BIO314H5 Lecture Notes - Lecture 11: Restriction Fragment Length Polymorphism, Dna Profiling, Alphonse Bertillon
Document Summary
Forensics- goals and investigations- why dna is so useful for this?- sequence proteins and remove them from the surface of different materials- mass spec in-situ. Previous times- person"s name changes = record left behind. Bertillon- series of measurements of face and body parts- combinations of measurements provides unique code for people. West brothers- claimed never been to prison before- twin brothers- one already in jail- problems of bertillon analysis- set base for facial recognition. Finger-print analysis- west brothers weren"t the same: can be left on the crime scene but can be avoided by wearing gloves. Dna- differentiate btw people except identical twins- unique aspect. Dna you start with is what you end with- sequence remains the same. Sensitive- pcr- don"t need very much dna- touch is enough. First dna profiling- richard buckland-unambiguously identifying dna- required a significant amount of dna to work- differentiate between sperm sample and victim"s dna- buckland was not guilty- exonerated an innocent person.
