BIOB10H3 Lecture Notes - Lecture 7: Van Der Waals Force, Affinity Chromatography, Differential Centrifugation

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27 Oct 2016
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Whe(cid:374) (cid:449)e"(cid:396)e taki(cid:374)g apa(cid:396)t p(cid:396)otei(cid:374), (cid:449)e (cid:374)eed to ha(cid:448)e the(cid:373) i(cid:374) thei(cid:396) sa(cid:373)e e(cid:374)(cid:448)i(cid:396)o(cid:374)(cid:373)e(cid:374)t (cid:271)(cid:455) keepi(cid:374)g them in their original structure. When extracting cell, they are kept in a controlled manner to keep them in their shape. We can pull protein apart due to their size and density. They would settle at different level because they have different size and pulled by gravity at different force. To speed up the process, it is centrifuged to settle the component faster. There is other type of things that are used to settle components like microfuge and ultracentrifuge. The supernatant can be decanted or pipetted and added to another tube and the process can be repeated. We use the same supernatant to centrifuge and break apart the organelle, the microsomes, and ribosome those kinds of stuff to take apart. The denser the component, the more they are dragged to the bottom. Different density of component will form different bands.

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