BIOB10H3 Lecture Notes - Lecture 3: Endoplasmic Reticulum, Secretion, Endomembrane System

95 views7 pages
28 Oct 2017
School
Course
Professor

Document Summary

Early em studies revealed organelles inside cells (cid:862)la(cid:272)e-like(cid:863) (cid:374)et(cid:449)ork spread throughout the (cid:272)ytoplas(cid:373: organelles: membrane bound cellular compartments w/ unique functions/proteins. Claude & palade labeled proteins w/ radioactive isotopes and detected them by autoradiography to find out more about the function of the endomembrane system. Isotopes: same elements have same number of protons but not neutrons. Radioactive isotopes (radioisotopes) contain unstable combo of proton & neutron: unstable atoms break apart or disintegrate to reach stability, disintegration causes release of energy in form of particles or electromagnetic radiation (radioactivity) Include radioactive amino acids w/ one or more radioactive atoms in the growth media of cells. Ribosomes insert radioactive amino acids into proteins just as they normally would. Autoradiography energy emitted from radioactive atoms used to label proteins can be used to activate a radiation sensitive emulsion. Silver grains will be visible in em images showing location of protein in cell.

Get access

Grade+20% off
$8 USD/m$10 USD/m
Billed $96 USD annually
Grade+
Homework Help
Study Guides
Textbook Solutions
Class Notes
Textbook Notes
Booster Class
40 Verified Answers
Class+
$8 USD/m
Billed $96 USD annually
Class+
Homework Help
Study Guides
Textbook Solutions
Class Notes
Textbook Notes
Booster Class
30 Verified Answers

Related Documents

Related Questions