BIOB10H3 Lecture Notes - Lecture 3: Endoplasmic Reticulum, Secretion, Endomembrane System

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28 Oct 2017
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Early em studies revealed organelles inside cells (cid:862)la(cid:272)e-like(cid:863) (cid:374)et(cid:449)ork spread throughout the (cid:272)ytoplas(cid:373: organelles: membrane bound cellular compartments w/ unique functions/proteins. Claude & palade labeled proteins w/ radioactive isotopes and detected them by autoradiography to find out more about the function of the endomembrane system. Isotopes: same elements have same number of protons but not neutrons. Radioactive isotopes (radioisotopes) contain unstable combo of proton & neutron: unstable atoms break apart or disintegrate to reach stability, disintegration causes release of energy in form of particles or electromagnetic radiation (radioactivity) Include radioactive amino acids w/ one or more radioactive atoms in the growth media of cells. Ribosomes insert radioactive amino acids into proteins just as they normally would. Autoradiography energy emitted from radioactive atoms used to label proteins can be used to activate a radiation sensitive emulsion. Silver grains will be visible in em images showing location of protein in cell.

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