BIOB11H3 Lecture Notes - Lecture 3: Escherichia Coli, Pribnow Box, Consensus Sequence
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Lecture 3: transcription i: overview and rrna synthesis/maturation. They reasoned that an organism with such broad synthetic capacity should be very sensitive to enzymatic deficiencies: their plan was to irradiate mole spores and screen them for mutations that caused cells to lack a particular enzyme. To screen for such mutations, irradiated spores were tested for their ability to grow in a minimal medium that lacked the essential compounds known to be synthesized by this organism. Results showed that a gene carries the information for the construction of a particular enzyme and this is called the one gene-one enzyme hypothesis. It was then learned that enzymes are composed of more than 1 polypeptide, each of which is encoded by its own gene, the concept (cid:271)e(cid:272)a(cid:373)e (cid:373)odified to (cid:862)one gene-o(cid:374)e polypeptide(cid:863). 11. 2 an overview of the flow of information in a eukaryotic cell: the dna of the chromosomes located within the nucleus contains the entire store of genetic information.
