BIOB11H3 Lecture Notes - Lecture 6: Northern Blot, Paper Towel, Rna Interference
Document Summary
Lecture 6: rna splicing, catalytic rnas and rna interference part 2. Sp 11. 34- schematic representation of a mechanism for the coordination of transcription, capping, polyadenylation, and splicing: blue is rna polymerase; red strand is rna. Its been going on: suppose that it just started (series of events). Once phosphorylation takes place, those are binding sites for some of the capping proteins. Capping and some of splicing can go on before rna polymerase finishes its job! Sequence for polyadenylation, an enzyme poly a polymerse sees that sequence and signals that we are close to end of molecule that precipitates the cutting of rna and addition of poly a tail to it. What happens: paper (cid:449)/ na + radioa(cid:272)ti(cid:448)e pro(cid:271)e put together i(cid:374) i(cid:374)(cid:272)u(cid:271)ator. Ne(cid:454)t da(cid:455), (cid:455)ou see (cid:272)ollisio(cid:374) (cid:271)(cid:449)/ radioa(cid:272)ti(cid:448)e pro(cid:271)e a(cid:374)d (cid:449)hat(cid:859)s o(cid:374) surfa(cid:272)e on membrane of paper: wash (cid:894)(cid:449)hat(cid:859)s (cid:374)ot (cid:271)ou(cid:374)d(cid:895), expose to xray film to detect.
