BIOB11H3 Lecture Notes - Lecture 4: Tata-Binding Protein, Rna Polymerase Ii, Tata Box
Document Summary
Transcription: mrna: mrna: messenger rna genetic message for translation to protein, synthesized from pre-cursor rna called heterogeneous nuclear rna (hnrna, pulse-chase experiment shows that hnrna is the precursor to the mrna. Tata binding protein (tbp), gtfs and the polymerase. 38 kda tbp and 13 tbp-associated factors (tafs: tbp inserts into the minor groove of the double helix and bends dna ~80o at the site of dna-protein interaction. Initiation of transcription: the assembly of the preinitiation complex occurs in a step-wise manner, tfiid tfiia and tfiib rna pol ii and tfiif . Tfiie and tfiih: tfiih assembles onto the complex once rna pol ii is bound. Tfiih has two enzymatic functions: helicase unwinds dna, kinase phosphorylates pol ii, tfiih is required for transcription initiation. Tbp binds to the promoter while other subunits of tfiid complex bind to other regions of dna.
