BIOB11H3 Lecture Notes - Lecture 11: Htc One X, Cas9, Nucleosome
Document Summary
Lecture 11: gene regulation iii: epigenetics & miscellaneous gene regulatory mechanism. Information ow through dna to rna to protein. Mechanisms that alter expression but which do not involve changes to dna sequence (basically no mutations) >often a functional group (e. g. methyl) is added/lost. Condensed depending on cell type, stage of development or other stimulus. During embryogenesis one, of the 2 x chromosomes in females in inactivated. *refer to lecture 8 for histone & nucleosome diagrams. Tail that extends from the dna in histones can be enzymatically modi ed by covalent addition of: Ubiquitin can bind to lysine in histones h2a/h2b in core. Modi cations affect af nity for tf that control transcription of chromatin. Enzymes are also used to remove these groups. Dicer and other rnai machinery (see lec 5) form it into single- stranded sirna and associate with protein complex. 4. ) sirna-protein complex bound to complimentary nascent rna recruit histone methyltransferase(hmt) suv39h1 to methylate k9 on h3 (replaces acetyl)
