BIOB11H3 Lecture Notes - Lecture 5: Tata-Binding Protein, Precursor Mrna, Tata Box
Document Summary
Lecture 5: hnrna synthesis and processing hnrna = precursor of mrna. Half life - half way point of synthesizing and destroying a molecule: hnrna is synthesized and broken quickly whereas rrna persists in cell for a long period of time, explains discrepancy in amount of rrna and hnrna. Sequences in promoter gene that tells rna polymerase where to bind: promoter is upstream from the gene it interacts with. Tata box is located 25 nucleotides upstream conserved sequence, -10 in bacteria is the location. Mrna features: 5" end has a cap = 5" end has a triphosphate group that is modified but remains phosphate (1/3 phosphate is trimmed and gdp is added) Technology = mrna that has a polya tail, add oligo dt primer to base pair with tail to get a template, use reverse transcriptase to make rna dna, insert into cloning bacteria to make clones.
