BIOB11H3 Lecture Notes - Lecture 13: Dna Supercoil, Hydrolysis, Bromodeoxyuridine
Document Summary
Single band of dna at the bottom of the gradient for n15. Take some cells that are uniformly labeled and add them to n14 and create various generations. Dispersive = first round of replication afterwards you get molecules at the hybrid density, after 2 rounds = get lighter, progressive enrichment of. Conservative = first round of replication afterwards you get 1 band at the top and 1 band at the bottom, 2nd round you get more light bands, 3rd round you get even more light bands. Semiconservative = first round you get 2 molecules at the hybrid density, 2 rounds = 2 bands at hybrid and 2 bands at the light density, 3 rounds = more light bands (6?: actual results. After 1 generation, molecules sediment at the hybrid density. 2nd round half the molecules at hybrid density and other half at light density = semiconservative: used e. coli.
