BIOC13H3 Lecture Notes - Lecture 7: Facultative Anaerobic Organism, Oxidative Phosphorylation, Pyruvic Acid

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BIOC13:(Lecture(8( ( Midterm(2(
Feb(7,(2018(
21#
Glycolysis(IV!
Energetic)Summary)of)Glycolysis)
By#the#end#of#glycolysis,#glucose#has#been#converted#into#2#pyruvates#and#2#
NADH,#yielding#-154#kJ/mol#of#free#energy#
2#ADP#have#been#converted#into#2#ATP,#using#62#kJ/mol#of#energy#
Means#there#is#a#net#release#of#-92#kJ#of#energy#that#dissipates#as#heat#
Essentially,#40%#of#the#free#energy#released#from#glycolysis#is#conserved#in#
ATP#making#it#highly#efficient#
However,#this#accounts#for#only#5%#of#energy#glucose#releases#when#fully#
oxidized#
o Forget#that#there#is#energy#stored#in#the#pyruvates#and#NADH#that#is#
released#via#other#processes#so#you#can’t#count#the#energy#glucose#holds#
but#the#energy#the#process#actually#releases#and#then#conserves#
Pasteur)and)Crabtree)Effect)
Facultative(anaerobes#do#both#glycolysis#and#oxidative#phosphorylation,#
depending#on#the#concentrations#of#glucose#and#oxygen#
Pasteur(effect(is#that#in#low#oxygen#environments#glycolysis#will#be#used#while#
oxidative#phosphorylation#is#used#in#high#oxygen#levels#
o When#oxygen#is#low,#need#to#use#
anaerobic#metabolism#(glycolysis)#
o When#oxygen#is#high,#rather#make#
more#energy#via#oxidative#
phosphorylation#
Crabtree(effect#is#the#switch#from#
oxidative#phosphorylation#to#glycolysis#
(or#vice#versa)#depending#on#glucose#
concentration#
o In#high#glucose#concentrations,#glycolysis#is#okay#to#use#since#it’d#use#up#
all#the#glucose#
§ Facultative#anaerobes#also#want#to#avoid#using#oxygen#too#much#
since#it’s#toxic#and#therefore,#if#there’s#enough#glucose,#glycolysis#
is#favourable#for#them##
§ Used#to#detect#cancer#as#glucose#would#accumulate#in#areas#with#
tumours#(they#don’t#do#oxidative#phosphorylation)#
o In#low#glucose#levels,#oxidative#phosphorylation#is#preferred#simply#
because#you#get#more#energy#with#less#glucose#(use#it#more#efficiently)#
Other)Sugars)in)Glycolysis)
Glycolysis#can#oxidize#other#monosaccharides,#but#there#are#extra#steps#
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