BIO130H1 Lecture Notes - Lecture 8: Cleavage And Polyadenylation Specificity Factor, Base Pair, Exon

• School: UTSG
• Department: Biology
• Course: BIO130H1
• Professor: Mitchell/ Moeder

Document Summary

Normal mrna is formed from three exons. Some single-nucleotide changes may destroy a normal splice site, causing exon skipping (eg. exon 2 may be missing) Some single-nucleotide changes may destroy a normal splice site, activating cryptic splice sites (eg. mrna with an extended exon) Some single nucleotide changes may create new splice sites casing new exons to be incorporated (mrna may have an extra exon inserted between two existing exons) A number of diseases are associated with exon skipping. Rna p(cid:396)o(cid:272)essi(cid:374)g to ge(cid:374)e(cid:396)ate the (cid:1007)" e(cid:374)d also (cid:396)e(cid:395)ui(cid:396)ed. Consensus sequences (in the dna, which are transcribed into rna; proteins that recognize se(cid:395)ue(cid:374)(cid:272)es i(cid:374) the rna(cid:895) di(cid:396)e(cid:272)t (cid:272)lea(cid:448)age a(cid:374)d pol(cid:455)ade(cid:374)(cid:455)latio(cid:374) of the (cid:1007)" e(cid:374)d (cid:894)euka(cid:396)(cid:455)otic mrnas ha(cid:448)e a lo(cid:374)g st(cid:396)i(cid:374)g of a"s(cid:895) These sequences are encoded by the dna to be transcribed. Transcription of the consensus sequences and recruitment of modifying proteins. Cpsf(a cleavage and polyadenylation specificity factor) and ctsf move from the ctd to specific sequences on the rna.