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Lecture 7

BIO130H1 Lecture Notes - Lecture 7: Phosphodiester Bond, Dna Replication, Sigma Factor

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Melody Neumann

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Week 5 - Transcription Part I
Lecture Outline:
1) Molecular definition of a gene
2) Transcription and RNA Polymerase in prokaryotes: bacteria
Readings: 428-433
Recall the flow of information in the cell
Molecular Definition of a Gene
The entire nucleic acid sequence (usually DNA) that is necessary for the synthesis of a
protein (and its variants) or RNA. In other words, genes are segments of DNA that are
transcribed into RNA. Remember there are two types of genes that when transcribed:
1.The resulting RNA encodes a protein
2.The resulting RNA functions as RNA
transcription (gene expression)
under the control of promoter sequences, which are generally upstream of the gene
the amount of RNA correlates to the amount of protein, but note that translation doesn't
always result in lots of protein
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