BIO130H1 Lecture Notes - Lecture 10: Release Factor, Peptide, Eef-1

• School: UTSG
• Department: Biology
• Course: BIO130H1
• Professor: Melody Neumann

Document Summary

Lecture outline: translation in prokaryotes and eukaryotes including quality control, introduction to protein folding. Recall the genetic code: codons read as mrna triplets, encoding all 20 amino acids, redundancy - with multiple codons for most amino acids. Reading frames define the amino acid sequence different reading frame will result in different proteins. Single-stranded molecule, but double stranded in some places through complementary base-pairing. The amino acid acceptor arm: bind to amino acid. The anticodon region: recognize its complement on the rna. More than 1 trna for many amino acids. Some trnas can recognize and base pair with more than 1 codon ) Recognition of a specific trna by its synthetase. Recognition is achieved by: identifying trna anticodon nucleotides, recognizing nucleotide sequence of acceptor stem, reading nucleotide sequences at additional positions on the trna. Overview of the catalytic process for amino acid addition. Energy stored in covalent bond between aa and trna in p position makes peptide synthesis energetically favourable.