BIO230H1 Lecture Notes - Lecture 10: Cytosol, Glycosylphosphatidylinositol, Lipid Bilayer
42
BIO230H1 Full Course Notes
Verified Note
42 documents
Document Summary
Can be further sorted by vesicular transport to other compartments or cell surface. Includes: soluble proteins, transmembrane proteins, proteins destined for golgi, secretion, lysosomes. Er signal sequence emerges first: hydrophobic amino acids at n-terminus, ribosomes directed to er membrane co-translational process. Signal recognition particle (srp) and srp receptor have gtpase domains: binds gtp. Srp and ribosome and er signal sequence have high affinity: srp and signal sequence causes pause in translation. Ribosome protein translocator channel (translocon: gated channel. Ribosome forms tight seal with translocator: prevent diffusion of ions and small molecules. Srp and srp receptor: gtp hydrolysis, complex dissociates. Er signal sequence has start-transfer sequence bound to translocator. Er signal sequence laterally diffuses into lipid bilayer: translocated gated in 2nd direction. Single-pass and multi-pass transmembrane (tm) proteins: n and c terminal ends sorted out during translocation. Single-pass tm proteins have three types of insertions.
