BIO230H1 Lecture 7: Lecture 7- Regulation of the Proteome
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BIO230H1 Full Course Notes
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Lecture 7- regulation of the proteome: translational regulation, post-translational regulation readings (alberts et al. , custom text) 15-29; 119-123; 82-91. Differences in the proteins expressed by two human tissues. Both prokaryotes and eukaryotes use translational control mechanisms to regulate protein expression, often in response to stressful situations such as low nutrients, infection, or environmental stresses (eg. temperature) Translational control usually used as a rapid response because the slower, the higher change it will be degraded. Mrnas have a six nucleotide shine-dalgarno sequence. Correctly positions aug in the ribosome and provides major translational control mechanism -- placing the ribosome at the right place to start translation (accessibility) Rna binding protein blocks access to sd sequence. Rna binding protein competing with ribosome to bind to the sd sequence. Rna adopt complex 2nd structure (eg. stem loop) - influenced by temperature usually destabilized by high temperature. Eg. virulence genes of human pathogen listeria monocytogenes (the genes are regulated at transcriptional level)
