BIO230H1 Lecture Notes - Lecture 6: Archaea, Veterinary Virology, Histone Methylation
31 Jan 2018
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BIO230H1 Full Course Notes
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Tuesday, september 26th, 2017 12pm-2pm (alberts et al. Lecture 6 outline: post-transcriptional regulation, mrna quality control, mrna stability. Rna processing: eukaryotic rna processing is initiated before transcription is complete. Prokaryotes are simpler, so it goes from transcription, to translation all in the same compartment. In eukaryotes, pre-mrna is produced by transcription in the nucleus (rna polymerase ii) The pre-mrna must be processed: 5" cap on 5" end, introns must be spliced out of pre-mrna, and a 3" poly-a tail sequence added on: only once these three processes have occurred will you have a mature. Rna that can be exported from the nucleus into the cytosol. This process of exporting rna into the cytosol is tightly control. It first binds the 5" capping factors (three enzymes) Phosphorylation of ctd of rna polymerase ii changes during transcription elongation: as it transcribes, phosphorylation pattern changes (different phosphorylation states, different states bind to different enzymes, middle phosphorylation state bind splicing proteins.
