BCH210H1 Lecture Notes - Lecture 15: Leucine, Valine, Alanine

• School: UTSG
• Department: Biochemistry
• Course: BCH210H1
• Professor: Reinhart Reithmeier

Document Summary

Proteins: integral (embedded in the membrane, peripheral (attached adjacently to the membrane) Removal of these proteins mild disruptions: high salt, small changes in ph. Ways of adhering to membrane: ionic & h-bonds, amphipathic -helix, has 2 different characteristic faces, motif: alternating polar-nonpolar residues, polar polar nonpolar nonpolar polar , hydrophobic loop (embedded into the lipids, association with integral protein. Removal methods: high salt, shielding charges disrupts ionic & hydrogen bonds, change ph, adds or removes h to the aa loss of interactions b/w residues, asp, glu, his, lys, arg, cys, tyr. Anchored, b/c completely spans the lipid bilayer: requires harsh conditions to remove from membrane. + on inside: type i- single pass nh3, type ii- single pass nh3, type iii- multi-pass, type iv- single pass w/ multiple proteins, type v- anchored by covalently attached lipids, type vi- transmembrane segments & lipid anchors. Removal/ isolation of protein: add detergents (ex.