BCH210H1 Lecture Notes - Lecture 9: Protein Structure Prediction, X-Ray Crystallography, Globular Protein

• School: UTSG
• Department: Biochemical Engineering and Applied Chemistry
• Course: BCH210H1
• Professor: Wright

Document Summary

Anfinsen experiments shown that info for 2 o and 3 o structure is coded in the aa. Folding code - the thermodynamic question of what balance of interatomic forces. Protein structure prediction - predicting the protein"s native structure by the aa sequence. Ramachandran plot dictates the structure of the protein for a given aa sequence. The (phi,psi) angle causes restriction as to how the protein can fold. Helix and sheets involve sequence in low energy areas of the plot. Other areas of the plot are higher energy due to steric clashes. Spectroscopic methods can only give us and estimate % of 2 o structures in protein, but doesn"t actually located exactly where the 2 o structures are. 30 globular protein structures solved by x-ray crystallography used. Each residue in each protein was assigned as occurring in an helix, sheets, turns or disordered region of the protein determined by its phi psi angles. Helix when consecutive sequences of 4+ helical residues.