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Lecture 5

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Jane Mitchell

Lecture 5 Introduction to DNA Replication1Overview of DNA replication2DNA replication in bacteriaIs DNA replication conservative or semi conservativesto1 optionIn a conservative model of replication both strands are conserved and wind up in the same cellThe replication event results in daughter strands delocalized in another cellndo2 optionSemi conservative replication involves the targeting of 1 parental strand and 1 daughter strand into each of the resulting daughter cellsIn each generation you are sending forward a parental strand that you know is correct that has been proofed So during the replication events any mistakes in the daughter cells are now going to be correctedWhat is the direction of DNA replicationsto1 optionUnidirectional growth of a single strand from 2 starting pointscopying the parental strand from the 3 endndo2 optionUnidirectional growth of 2 strands from 1 starting pointrdo3 optionBidirectional growth from 1 starting pointmeans that where the site of initiation of replication occurs you wind up with the bidirectional growth occurring in both directionsWhere does DNA replication startsto1 optionAlways start from the same location on DNAWe need a region of the DNA that is easily separated into single strand and you can maximize the chances by modifying the base configurationmore A T residues are easier than G C richThey have to have sufficient sequence diversity in order to be recognized and bind to initiator complexndo2 optionRandom locationHow many origins of replicationoBacteria are usually single replicationThey are located at particular region on circular chromosome1 replication origin per chromosomeThere are specific sense of ques environmental sensors that say its time to growHighly controlled event because of little growth in bacteriaoEukaryotes are multiple replicationMultiple genomesThe ones in red are nondefault and the green parts are the regions that are usually replicatedIf the environment is good the yeast will replicate in all of the originsAn experiment to identify origins of replication in yeastoOrigins of bacteria are also found on plasmidsoAs long as you got the origins of replication on that plasmid thats specific for that organism that plasmid will replicate autonomously inside that bacteriaoIn bacteria you can determine origins of replication by looking for specific sequences on plasmids taking components of the chromosome and inserting into the plasmid and looking to see whether or not it will support etc
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