CSB349H1 Lecture 1: Section 1- L1b
14 May 2018
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Lecture 1(b): Genome Repeats
Polymerase Chain Reaction (PCR):
• Allows to measure the copy number (# of repeats) in DNA – tandem repeats
• Primers are complementary to the non-repetitive part of the genome (outside of microsatellite)
o Blue box displays the “CA” repeat on the chromosome
o Left and right of the blue box area are two primers in the unique part of the DNA
§ PCR experiment will amplify the region between the two primers which can
be run on a gel to measure the length of that region
• Two people in a population (person A and person B)
o Person B has one allele that contains CA repeats
o Person A has two alleles that contains CA repeats
ð Person B corresponds to allele #2 as the gel runs at 108 bp
o Primers began 40bp away from the repetitive region; therefore,
40 on each side = 80, 108 – 80 = 28, 28/2 = 14 repeats of “CA”
ð Person A corresponds to allele #1 and #3 as the gel runs at both 112 bp and 102 bp
*Gel displays 3 bands for each allele due to PCR errors in amplifying the region correctly
