HMB310H1 Lecture Notes - Lecture 3: Organ Culture, Collagenase, Cell Culture
Document Summary
Tissues culture: removal of cells, tissues or organs from an animal or plant and their subsequent placement into an artificial environment conducive to growth. In vitro culture (maintain/proliferate) of cells, tissues or organs. Moving from in vivo to in vitro: pros. A good starting point for cell biology research. Drastically reduces the use of animals: cons. Lacks several systemic components (homeostatic regulation via nervous and endocrine systems) Generalization of experimental results to in vivo conditions. Cell dissociation from 3d environment (to 2d) Cost of production of cells is 10 times higher than direct use of animal tissues (control of environment, infrastructure) Organ culture: entire embryos or organs are excised from body and culture that allows differentiation and preservation of architecture (~3d culture) Explant culture: small pieces of tissue are grown in culture medium, pros. Low quantities of the reagents required in contrast to in vivo studies: cons.
