IMM250H1 Lecture Notes - Short-Chain Fatty Acid, Segmented Filamentous Bacteria, Operational Taxonomic Unit
Document Summary
February 12, 2013 the microbiome in immune system development, function and disease. Diversity of genes of microbiome >>>>> human genome. Large scale projects to map human microbiomes . Metagenomics without prior culture these organisms discussed today are difficult to culture, does not like to live as single organisms. Probiotic do not colonize humans; come largely from cows and non-humans; cultured in labs; not part of humans, go through us. Prebiotics foodstuffs promote growth of beneficial bacteria . High through put sequencing method of choice when confronted with the diversity. 16s ribosomal gene all bacteria have this gene. Moutains and valleys of diversity make sets of oligonucleotide primers based on conserved regions of gene, pcr amplify, make libraries of amplicons; these amplicons include more variable regions where the sequences used to distinguish dna. Map of diversity in human microbiome sequence microbiota from different sources from internal and external body.
