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IMM334 - 1.docx

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Department
Immunology
Course
IMM250H1
Professor
All Professors
Semester
Fall

Description
IMM334Developmental ImmunologyFall 2013FIRST LECTURESRCSCID repopulating cells Increase frequency of SRC by using limiting dilution assay intrafemoral injection and SCIDNOD miceNODSCID repopulating cells SRC within the CD34CD38 population Ex vivo lentiviral transduction with GFP CB cells incubated with vector Day 1mice injectedcells grew better in engraftment Day 4cells and vector incubated with cytokines then injectedculturing led to fewer SRCsFACSHuman colony forming cell assay In vitro assay based on progenitors ability to proliferate and differeniatte into colonies in solid meadia methylcellulose based in response to cytok
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