MGY377H1 Lecture Notes - Lecture 26: Prophage, Wild Type, Chromosome
1 Feb 2018
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It identifies the nda, sees that its foreign, binds and shuts it down. Bacteria are using their specific gc contents to distinguish self from nonself dna. We can knock out the hns gene, and we use microarrays which allow us to look at the transcript of the entire genome all at once and mreausre transcript elvels. We look at wildtype sammonella and the array from salmonella without the hns gene. Hns is going down, activated by hns is going up. The most strongly downregulated by hns are associated with the gray blocks, and these genes are known to be acquired by salmonella via horiznontal ge(cid:374)e t(cid:396)a(cid:374)sfe(cid:396). O(cid:396)a(cid:374)ge is p(cid:396)ophage ge(cid:374)o(cid:373)es, hns does(cid:374)"t i(cid:374)flue(cid:374)(cid:272)e the(cid:373) (cid:373)u(cid:272)h. Hns could be a dna binding protein that binds a small spot on the chromosome which triggers transcription factors. How do we know hns is a direct factor. We treat the cell with frmaldehyde, and it crosslinks proteins to dna.
