MGY377H1 Lecture : MGY 377
26 Oct 2011
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Document Summary
If you have a sequence of dna, and you have a primer: you add in a dna polymerase, and it would start to add nucleotide into the strand. If you add a special dcp, and the strand can not extended: this strand would be label with raidoactive or florescent. If you follow the flow chart, how the enzyme use pp to remake atp and then another enzyme make light from atp, then you can read off the light. If you repeat the cycle, you will see different light emitted: each reaction you run with one dntp (n can be a, c, t, g, so as a result, you can see different light emitted. If you make it low enough concentration, you will have one bead stuck with one. If we can harnest bacteria, we can extract lot more for our own health. If you eat high fat diet for a long time, you will switch the dominate bacteria/enterotype.
