MGY440H1 Lecture Notes - Paraffin Wax, Pdz Domain, Respiratory Tract
3 Jun 2013
School
Department
Course
Professor
Document Summary
H5n1 virulence determinants c-terminal zielecki et al paper. Polybasic cleavage site in ha cleavage by furin-like proteases. Polymorphisms: all three above contribute to high virulence of h5n1. Pdz domain interact with ns1: may disturb function of various cellular protiens thus disease = hypothesis. Vn/1203 isolated from human fatal case. Use reverse genetics: produce recombinant vn/1203 virus, ns1 cterminal either avian esev or human rskv, three variants: truncated, rsev, esev. Conclusion: pdz domain ligand sequence (esev or rskv) in ns1 contributes little to the virulence of. A three viruses with different: rskv changed ns2 protein at position 70 changed amino acid at this position. Presence of pl motif reduces capacity of ns1 protein to support viral replication. Intracellular location of ns1 protein in transfected and infected cell cultures. Little ns1 in nucleus at all time points rskv in a549. Cef and def: wt little in nucleus; mainly cytosolic, esev nuclear accumulation of ns1 is controlled in avian cells.
