PSL350H1 Lecture 3: Lecture 3- Chromatin and Gene Regulation.pdf
Document Summary
Lecture 3: chromatin and gene regulation. Transcriptional regulation: protein can regulate the translatability of rna. Can bind and inhibit its translation, or super- activated: mrna can also feedback and inhibit, but never turn into protein. 3 billion bases, 2 metres. Short range organization (1- 100s kilobases) Long range organization (1- 100s megabases) Dna, organized as chromosomes. Wound as fibres on histones: histone cores dna is wrapped around it. Epigenetic labels covalent modifications to the histones and dna. Discrete elements: open chromatin: promoter (transcription initiation rna polymerase ii binds and starts making mrna) Crm- cis regulatory module: cis regulatory molecules: places where other protein factors can bind. Cis = aligned with there the transcriptional start site is. Tfbs - transcription factor binding site. Where did the histones go: dna not exactly wrapped tight around histone core enzymes wont be able to access. Gene expression enhancers and promoters: winding and unwinding dna process. Chromatin architecture in gene expression.
