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Lecture

Gel Electrophoresis

2 Pages
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Department
Biochemistry
Course Code
Biochemistry 2280A
Professor
Eric Ball

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Description
Gel ElectrophoresisHow do you identify and resolve dna moleucles You cut dna with restirction enzyme and want to see what fragments u get They way you resolve dna is to resolve based on size All dna have very similar mass to hcarge ratio Way most dna differ is in their length separation of dna molecules is most esasily done on the basis of length of base pairs If two dna is same size its hard to separateMass to charge ratio is much the same for all dnabut length is diffif two dna have two exact size its hard to separateWhen placed in electric field at pH 7 DNA will migrate toward which poleA bc bothDna is negative at neutral pH due to phos back bone ve so it will move toward ve poleGel electrophoresis way for ppl to most comonly separate dna Its a sieving process For gel eelectrophoresis dna is sieved through a matrix of agarose or acrylamide which contains minute poresForce supplied to DNA is electric fieldput gel in electric field and electric field will cause DNA to move Using batteries or other power suorceRestriction enzymes cleave th
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