Class Notes (866,995)
CA (523,442)
Western (51,171)
Biochemistry (862)
2280A (649)
Eric Ball (118)

Gel Electrophoresis

2 Pages

Course Code
Biochemistry 2280A
Eric Ball

This preview shows 80% of the first page. Sign up to view the full 2 pages of the document.

Loved by over 2.2 million students

Over 90% improved by at least one letter grade. are saying about us

Leah — University of Toronto

OneClass has been such a huge help in my studies at UofT especially since I am a transfer student. OneClass is the study buddy I never had before and definitely gives me the extra push to get from a B to an A!

Leah — University of Toronto
Saarim — University of Michigan

Balancing social life With academics can be difficult, that is why I'm so glad that OneClass is out there where I can find the top notes for all of my classes. Now I can be the all-star student I want to be.

Saarim — University of Michigan
Jenna — University of Wisconsin

As a college student living on a college budget, I love how easy it is to earn gift cards just by submitting my notes.

Jenna — University of Wisconsin
Anne — University of California

OneClass has allowed me to catch up with my most difficult course! #lifesaver

Anne — University of California
Gel ElectrophoresisHow do you identify and resolve dna moleucles You cut dna with restirction enzyme and want to see what fragments u get They way you resolve dna is to resolve based on size All dna have very similar mass to hcarge ratio Way most dna differ is in their length separation of dna molecules is most esasily done on the basis of length of base pairs If two dna is same size its hard to separateMass to charge ratio is much the same for all dnabut length is diffif two dna have two exact size its hard to separateWhen placed in electric field at pH 7 DNA will migrate toward which poleA bc bothDna is negative at neutral pH due to phos back bone ve so it will move toward ve poleGel electrophoresis way for ppl to most comonly separate dna Its a sieving process For gel eelectrophoresis dna is sieved through a matrix of agarose or acrylamide which contains minute poresForce supplied to DNA is electric fieldput gel in electric field and electric field will cause DNA to move Using batteries or other power suorceRestriction enzymes cleave th
More Less
Unlock Document
Subscribers Only

Only 80% of the first page are available for preview. Some parts have been intentionally blurred.

Unlock Document
Subscribers Only
You're Reading a Preview

Unlock to view full version

Unlock Document
Subscribers Only

Log In


Don't have an account?

Join OneClass

Access over 10 million pages of study
documents for 1.3 million courses.

Sign up

Join to view


By registering, I agree to the Terms and Privacy Policies
Already have an account?
Just a few more details

So we can recommend you notes for your school.

Reset Password

Please enter below the email address you registered with and we will send you a link to reset your password.

Add your courses

Get notes from the top students in your class.