You are interested in how expression of YFG1 is regulated inseveral different cell types. You have discovered that there arethree different regions of the YFG1 promoter (A, B and C) that areimportant in regulating gene expression in different cell types.Below is a schematic of the gene for YFG1 and its upstreamregulatory regions: see figure
You decide to investigate the role of these three regulatoryregions by cloning the DNA sequence upstream of YFG1 and fusing itto a reporter gene.
A) What reporter gene would you use if youwanted to read out expression using fluorescent microscopy?
After making the reporter construct you decide to mutate thedifferent regulatory regions in order to determine their relativecontributions to the expression of YFG1 in the cell types indicatedbelow. Your data is summarized in the table below: Regulatoryregions present (not mutated): See table
B) Does sequence A activate expression of YFG1or repress expression of YFG1?
C) You find out that protein Z is atranscription factor that binds exclusively to sequence C. Isprotein Z a transcriptional activator or a repressor? In whichtissues would you expect to find protein Z expressed?
D) You find out that protein Y is atranscription factor that binds exclusively to sequence B. Isprotein Y a transcriptional activator or a repressor? In whichtissues would you expect to find protein Y expressed?
Table and figure:
<a href="http://imgur.com/SImKlva"><imgsrc="http://i.imgur.com/SImKlva.png" title="source: imgur.com"/></a>