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Lecture

LC-MS-MS Diagram.docx

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Department
Biochemistry
Course
Biochemistry 3386B
Professor
L.Graham Smith
Semester
Winter

Description
High Pressure Liquid Chromatography/Mass Spec/Mass Spec (LC/MS/MS) AKA: LC Tandem MS or Triple Quad LC/MS Free ions (no solvent) Sent to Mass Spec The ions are getting closer ElectrosprayInterface: together and start to repel between HPLC and MS which gets rid of most one another, blows apart the of the solvent and fragments and ionizes the analyte moleculesentering the first drops as they get smaller Quadropole Like the GC/MS the LC/MS/MS First Quadropole can be used in toxicology typically set to allow onlyonefragment ion applications. from the MS of the analyte Analyzed based on retention time (of the component peak) and characteristic ions (mass spec) The whole process is refer
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