Biochemistry 2280A Lecture Notes - Lecture 28: Reverse Transcriptase, Nitrocellulose, Transfer Rna
12 Dec 2016
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Topic 25 continued: recombinant dna technology case study. You"ve identified a human protein that will be of tremendous value in treating disease. You"re goal is to mass produce this protein (yfp = your favourite protein) Many proteins are difficult to obtain from their native source (usually not enough proteins in the tissue, or the tissue is rare) Rdt can dramatically increase expression and facilitate purification = you can get to the source tissue more easily: process of expressing yfp requires: Purifying the protein from e. coli: bacteria (e. coli) are most commonly used to express yfp. Easy to physically break then open and release the protein (some organisms have cell walls that are hard to break but bacteria walls are easy to break) Multi-copy plasmids and strong promoters can drive expression. Expressing yfp in e. coli (in the workbook: the first issue we need to think about is that we need a source of the gene.
