Biochemistry 2280A Lecture Notes - Lecture 22: Hbb, Consensus Sequence, Polyadenylation
24 Dec 2016
School
Department
Course
Professor
Document Summary
Pre-mrna mrna: 5" capping, 3" polyadenylating, splicing. 7-methylguanosine attached at the end of the 5" end: this is often called the 5" cap, 5"-5" triphosphate linkage. Importance of the 5" cap: marks the 5" end of the mrna as being intact, required for, mrna export from the nucleus, translation of the mrna. 3" polyadenylation: mg: almost all eukaryotic mrnas end with a long (~300 base) polya tail, after the stop codon uaa, aauaaa recruits complex which cleaves the rna and then signifies adding a. Polya tail (adding around 300 bases on: importance of the 3" polya tail, marks the 3" end of the mrna as being intact, protects the mrna from degradation, required for. Acts as a buffer at the 3" end where nucleases trying to degrade the rna. The cell requires both polya tail and the 5" cap to get moved into the cytoplasm: cell regulation: only having one indicates clipping and should not be moved for translation.
