Biochemistry 2280A Lecture Notes - Lecture 24: Primary Transcript, Intron, Polyadenylation

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Topic 24: rna processing in eukaryotes lecture notes. When a transcript is first made, it ends with a triphosphate. First nucleotide that comes in is a triphosphate. Happens as soon as the rna is exposed in eukaryotes, a 7-methylguanosine is added to that linkage is a 5"-5" linkage (unique) Marks the 5" end of the mrna as being intact. Required for: mrna export from the nucleus, translation of the mrna. Primary transcript would still have rna polymerase working. After the stop codon, there is a sequence that resembles aauaaa. Recruits an enzyme that clips the rna 30 bases downstream and then. String of a bases can be as long as 300, very long tail at the end of the. Happens with transcription ongoing in eukaryotic cells adds a long string of a bases mrna. Marks the 3" end of the mrna as being intact. Protein encoding sequences (exons) are interrupted by noncoding sequences (introns)

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