Biochemistry 2280A Lecture Notes - Lecture 5: Ethidium Bromide, Recombinant Dna, Sticky And Blunt Ends
14 Dec 2017
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Section 5 recombinant dna technology and genomics. After completing this topic, you should be able to: describe the tools of recombinant dna technology and explain how each function. Dna is a long relatively homogeneous polymer (it all looks alike) Restriction enzymes cut dna into defined workable units (manipulate the dna) Can be though of as site specific scissors. Site specific dna binding proteins that bind to and recognize and cleave palindromic. Dna sequences of 4, 6 or 8 base pairs. Palindromic dna sequences: have a 2 fold symmetry. Can tell where the cut is based on the sequence. Some restriction enzymes cut identical sequences but leave different types of ends. Some restriction enzymes cut different recognition sequences but leave identical overhanging ends. Restriction enzymes are enzymes so like any enzyme each has a preferred condition in which it functions: like temperature, ph and salt conditions.
