Biochemistry 2280A Lecture Notes - Lecture 23: Craig Venter, Genomic Library, Restriction Enzyme
30 Dec 2017
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Genomics: can tailor treatment to your disease, or look at your susceptibility to disease based on your genome. Sequencing genomes: craig venter & francis collins, classical genome sequencing involves, creating a genomic dna library, many independent sequencing reactions, aligning the independent sequences into a continuous sequence. Genomic dna library: definition: a collection of cloned dna fragments that represent all of the dna in an organism"s genome cdna library represents all of the rna. Lots of cells- many copies of the genome. Fragment with restriction nuclease (partial digest= chunks of intermediate size: millions of genomic dna fragments, dna fragments inserted into plasmids using ligase= recombinant dna molecules, all the inserts represent all of the genome. Transform plasmids into bacteria to produce lots of clones. Influenza, a gram negative bacteria, genome is circular. Start with millions of cells: create genomic library, extract dna, dna fragments of various sizes- ran on gel electrophoresis to purify the dna fragments of desired.
