Biochemistry 2280A Lecture Notes - Lecture 10: Peripheral Membrane Protein, Lipid-Anchored Protein, Lipid Bilayer
11 May 2020
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Document Summary
Hydropathy plots can be used to localize potential a-helix segments in the membrane, and calculate the hydrophobicity of successive segments. Single pass type 1, the end terminus is outside the cell (extracellular). Single pass type 2, the c-terminus is extracellular (opposite way round) Peripheral membrane proteins: associate with integral membrane proteins, contain lipid anchors, don"t penetrate lipid bilayer, can be intracellular or extracellular, intracellular with lipid anchor, extracellular with lipid anchor, intracellular binds to tm protein, extracellular binds to tm protein. More than 200 types; happen once the protein has been made. Changes in the primary structure of a protein, controlled by amino acids. Lipid modifications - aim to allow peripheral membrane proteins to interact with the membrane by a variety of lipophilic covalent attachments that help to bind proteins. Lipid modifications can also occur to integral membrane proteins. Intracellular: acetylation - the addition of myristyl or palmitoyl groups, prenylation the addition of farnesyl or geranylgeranyl groups.
