Class Notes (838,343)
Canada (510,861)
Biology (6,824)
Biology 1002B (1,346)
Lecture 8

Lecture 8.docx

1 Page
Unlock Document

Biology 1002B
Denis Maxwell

Lecture 8: integrated Metabolism 1. Change in respiration rate (oxygen consumption) in isolated mitochondria by NADH, ADP, uncoupler  Add mitochondria to an oxygen electrode chamber o Rate of 2 consumption measures rate of respiration o Amount of oxygen decreases o Isolated mitrochondria have no source of substrate  Disconnected from glycolysis so no pyruvate  Quickly deplete NADH  Add NADH at 4 minutes o The rate of the electron transport should go up o Rate of consumption of2O decreases even faster o Adding substrate (NADH)  Add ADP at 6 minutes o Rate of consumption of2O ↓ even faster o Becomes harder and harder to pump protons if proton + ADP  ATP doesn’t occur  No ADP in isolated mitochondria then low pH becomes very low since lots of protons  Add uncoupler at 8 minutes o Rate of consumption of O2 ↓ at fastest (highest rate) o There’s no more respiratory control o No proton gradient because H+ just diffuses through o Very easier to oxidize NADH and pump protons 2. Definition of respiratory control and how it is accomplished (proton gradient).  Regulatory mechanism o controls rate of ETC according to need via a direct influence of electrochemical proton gradient o ADP controls rate of ET  the lots of NAD
More Less

Related notes for Biology 1002B

Log In


Join OneClass

Access over 10 million pages of study
documents for 1.3 million courses.

Sign up

Join to view


By registering, I agree to the Terms and Privacy Policies
Already have an account?
Just a few more details

So we can recommend you notes for your school.

Reset Password

Please enter below the email address you registered with and we will send you a link to reset your password.

Add your courses

Get notes from the top students in your class.