Biology 2382B Lecture Notes - Lecture 7: Hoechst Stain, Bud Bud, Spindle Apparatus

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Lecture 7/8 - mitosis and cell cycle control. Important that conditions are optimal in preparation for division. Cell cycle is ordered sequence of events in which a cell duplicates its chromosomes and divides into two genetically identical cells (under perfect conditions) G1, s, and g2 phases make up up interphase. Where most cells arrest when not not dividing (go) postmitotic cells. Preparation for chromosome segregation and cell division (~4 hours) Prophase: nuclear envelope breaks down, spindle apparatus forms, chromosomes condense. Metaphase: chromosomes align in plane center of cell. Anaphase: sister chromatids separate, pulled towards spindle poles. Use hoechst stain technique to stain dna in the nucleus to determine the dna intensity during cell division. Important to have an equal number of chromosomes distributed between daughter cells. Aneuploidy (unequal number of chromosomes) often leads to cancer. Dna content is proportionate to the amount of hoechst stain.

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