Biology 2382B Lecture Notes - Lecture 4: Secretory Protein, Secretion, Nuclear Membrane

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Cell biology lecture 4: protein synthesis and transport. Typical mammalian cell: 10,000 proteins, must be localized correctly, newly made peptides must be directed to the correct destination. Targeting: direct proteins to the right destinations (organelles, during or after synthesis. Sorting: direct proteins to the secretory pathway using tags (er, golgi, lysosomes) Er): those which reside in er and proteins which are sorted to pm, Golgi complex, and lysosomes: accordingly, two major protein-sorting pathways are known: nonsecretory and secretory, nonsecretory: processed inside the er and golgi but retained inside the cell. Sds-page is used to identify newly translated proteins. Proof secretory need to get through the er before they get outside of the cell: what are the important organelles for secretion. Proteins that are secreted have to go through rer. Second experiment: throw everything in together, mrna, ribosomes, and microsomes there was incorporation of mature protein within the lumen of the microsomes cotranslational transport.

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