Biology 2382B Lecture Notes - Lecture 2: Tubulin, Microtubule, Axoneme

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Topic 1 microtubules there is no empty space in cells the cytoskeleton is dynamic. Microtubule structure: hollow tubes, composed of dimers made of and tubulin (monomers are 55kda, 25nm diameter (singlet), Binds gtp only, while can bind gtp or gdp: hydrolyzes. Microtubule-associated proteins (map"s: map"s can alter microtubule stability, contain microtubule binding domain and projection domain. length of the projection domain determine distance between microtubules. Map2 has long projection and tau has short projection: phosphorylation tends to inactivate map"s and destabilize microtubules, +tip"s are map"s associated with (+) ends of microtubules. Microtubule end binding and severing proteins: kinesin-13 uses atp hydrolysis to rip dimers off the ends of microtubules, stathmin promotes gtp hydrolysis in -tubulin cap, promotes disassembly of microtubule, inactivated by phosphorylation. Microtubule motor proteins kinesin directed towards the (+) end. 2 heavy chains head, neck, and stalk: atpase activity and microtubule binding ability.

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