Biology 2581B Lecture Notes - Lecture 11: Thomas Hunt Morgan, Alkylation, Selectable Marker
13 Jun 2017
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Early we relied on mutants which either occurred spontaneously or were induced random: very rare, now we like to generate mutants with a specific mutation in a specific place. Often spontaneous and often morphological mutants: e. g. Arabbidopsis, found in 1873: sequenced in 1990 transcription factor coded which is regulated in flower organ development, e. g. The problem with spontaneous early mutants: spontaneous, too few, too slow and we need mutants other than morphological, in biochemical pathways, subtle changes not so obvious, timing. Induced mutants: solved this since we increased mutations in frequency and larger variety. Ems was highly used for inducing mutagens since it was: soluble, can be easily taken up by cells. Note: that these are essential things that are needed for a mutagen. E. g. either (grrgrr, ughugh) or (grrgrr,ughugh: when performing the cross, if not linked will be independently crossed, this will result in heterozygous.
