Biology 2581B Lecture Notes - Lecture 11: Southern Blot, Transposable Element, Ampicillin
25 Feb 2014
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Early on, we relied on mutants which either occurred spontaneously or were induced random: nowadays, we like to generate mutants with a specific mutation in a specific place, if possible. Early mutants: often morphological mutants, often spontaneous. Grist in glasgow: lack a thymus and cannot generate mature t lymphocytes. Therefore, they are unable to mount most types of immune responses: the genetic basis: disruption of the foxn1 gene, c. elegans, 1974 first suggestion to use this as a model organism. Problem: spontaneous too few too slow and wee need mutants other than morphological in biochemical pathways, with subtle changes: induced mutations = increased frequency and larger variety. Ease of use: soluble, can be taken up by cells. You start to screen individuals for morphological phenotypes, biochemical mutants, etc. You can use assays to screen individuals for developmental mutants, for mutants with different behavior patterns, etc.
