Biology 2581B Lecture Notes - Lecture 10: Nonsense Mutation, Zebrafish, Intron
1 Mar 2019
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Document Summary
Enhancers: protein binding sites that in associated with promoters can stimulate gene expression (enhance/stimulate gene expression: bend and bring regions in physical contact at the promoter. Promoters: position dependent, can"t move them around a lot. If you inverted a promoter it would no longer drive its normal gene. If you inverted an enhancer, it could still work, form more complicated loop. Insulators: regions that put a box/fence around the influence of enhancers: provide a way to regulate enhancers, so that the enhancer isn"t affecting every possible gene. Dracula gene: contributes to heme biosynthesis in zebrafish, g to t transversion. Mrna from mutant is longer than the wildtype: cdna from mutant is longer, mrna from mutant is longer = message is longer but the protein product is shorter. Snp that converts g to t abolishes splice signal, intron is not spliced: uaa codon becomes in-frame and prematurely stops transcription, premature stop codon.
