Biology 1002B Lecture Notes - Lecture 8: Electrochemical Gradient, Pyruvic Acid, Heterotroph
8 Feb 2013
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Lecture 8: integrated metabolism: change in respiration rate (oxygen consumption) in isolated mitochondria by nadh, adp, uncoupler. Add mitochondria to an oxygen electrode chamber: rate of o2 consumption measures rate of respiration, amount of oxygen decreases. Add nadh at 4 minutes: the rate of the electron transport should go up, rate of consumption of o2 decreases even faster, adding substrate (nadh) Add adp at 6 minutes: rate of consumption of o2 even faster, becomes harder and harder to pump protons if proton + adp atp doesn"t occur. No adp in isolated mitochondria then low ph becomes very low since lots of protons. Chlamydomonas"s mitochondrial atp production is the same as in animal cells. Atp doesn"t leave chloroplast since it is used up: adp goes to calvin cycle, other reaction such as synthesis of fatty acids, pigments, amino acids, calvin cycle consume atp. Autotrophs e. g. chlamy: use only carbon dioxide from calvin cycle to mitochondria to make its own food.
