Biology 1002B Lecture Notes - Lecture 10: Mixotroph, Cell Membrane, Acetyl-Coa
15 Apr 2017
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Isolating intact mitochondria = rate of cellular respiration. Measures the change in oxygen consumption by changing the mitochondria function. No electron transport: add washed mitochondria, add nadh, add adp and pi, add uncoupler (2,4-dnp) You essentially put holes all around the membrane and nothing stops the protons form rushing back through the membrane. Essentially puts holes all over the membrane so proton gradient can go right back through the membrane. No substrate for atp synthase, protons can"t move across. Restricts the rate of electron transport = respiratory control. Slow if no substrate for atp synthase (lower rate) Becomes harder and harder to pump protons because there"s no way to equal out the proton gradient. G3p in chloroplast is linked to cellular respiration. Chlamy has two organelles that can make atp (mitochondria and chloroplast) Can grow heterotrophically in dark and photosynthetically in light means it is mixotrophic metabolism. There is no glucose transporter on the plasma membrane.
