Biology 1002B Lecture Notes - Fall 2018 Lecture 11 - Enzyme kinetics, Competitive inhibition, Kilogram

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21 Sep 2018
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Gas exchange (rate of exchange of co2 and o2; can measure co2 xation or o2 production) easy with isolated chloroplasts. Cellular respiration is occurring simultaneously in chlamydomonas, so the e ect of cellular respiration must also be considered (uses oxygen) Rate of co2 xation = micro mol co2 consumed /cell /min. # micro mol co2 consumed per cell per minute is the rate of co2 xation. If the rate of co2 xation is negative, that means that co2 is being produced! Measure the rate of co2 xation when the light is zero (no light reactions/ calvin cycle co2 increases, xation rate is negative) Linear relationship under light limiting region (amount of nadph and atp are proportional to amount consumed in calvin cycle) In colder temperatures, the saturated rate would be reduced because the calvin cycle couldn"t turn over as fast. On this curve, mitochondria will always have a rate of -8.

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