Biology 1002B Lecture Notes - Lecture 10: Restriction Enzyme, Channelrhodopsin, Optogenetics
20 Jan 2020
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Document Summary
Possible sources of genes (ie. channelrhodopsin) for use in making transgenic organisms: chlamy genome, harvest mrna from chlamy and turn it into cdna, dna synthesizer; machines that manufacture dna sequence. Role of restriction endonuclease enzymes in creating recombinant dna: restriction endonuclease enzymes recognize specific. Dna sequences and make cuts; these pieces of dna can now be inserted into another dna molecule to make recombinant dna. Basic mechanism for creation of cdna: take mrna out of chlamy and reverse transcribe it using reverse transcriptase, creating double stranded dna called cdna (complementary dna) Role of plasmid (expression) vectors in genetic engineering: Role of antibiotic resistance genes on vectors in genetic engineering: cells that take up the vector will be resistant to. Ampicillin: cells that do not take up the vector will die when placed in an antibiotic rich environment. Mechanisms for introducing transgenes into microbes, plants and animals: Role of various dna sequences in "chr2eyfp" construct discussed in class:
